Molecular study of Enterobacter cloacae isolated from leukemia patients

Introduction and Aim: Polymerase chain reaction (PCR) of 16S rRNA virulence genes associated with Type Three Secretion System (TTSS) has been used as a rapid method for the identification pathogen Enterobacter cloacae in leukemia patients. Rapid diagnosis this becomes necessary starting proper treatment these In current study, we aimed to isolate from patients study TSSS isolates using molecular methods. Materials Methods: E. identified biochemical tests was confirmed by polymerase (PCR). Genomic DNA extracted also subjected escV ascV specific primers. Results: 30 (23.07%) out 130 blood samples tested were cloaca tests. Further confirmation methods showed only 11 be positive. Few positive TTSS ASCV gene presence gene. All strains negative Conclusion: PCR is best technique comparison other conventional because its safety, high sensitivity, specificity, speed.